Isolation of Rutin from Phyllanthus Amarus

General phytochemical screening of Phyllanthus amarus (Euphorbiaceae) revealed the presence of Saponin, tannins, glycoside and alkaloids etc. The aim of this study is to identify and characterize the bioactive principle from the plant. It has wide folk medicinal uses. The isolation and characterization of Phytoconstituents was done from the methanolic extract by the gradient fractionation method. The structure of the isolated compound was established on the basis of physical, chemical test and spectroscopic evidences (IR, UV, HNMR, MS). A flavonol structure was isolated from the methanolic extract of the plant. The odorless, colorless compound which solubilizes in methanol having m.p. 186C and Rf value 0.46 in mobile phase Methanol: Glacial Acetic Acid: Water (90: 5: 5) was identified by the various spectroscopical methods. So from this study it is concluded that isolated compound may be rutin which is responsible for various pharmacological activities of the plant. INTRODUCTION: The plants of the genus Phyllanthus (Euphorbiaceae) are widely distributed in most tropical and subtropical countries. It is a very large genus consisting of approximately 550 to 750 species and is subdivided into 11 subgenera. Phyllanthus genera contain many important phytoconstituent which are responsible for various type of pharmacological activity like very effective hepatoprotective agents in the Indian indigenous systems of medicine and are considered bitter, astringent, stomachic, diuretic, febrifuge, deobstruant and antiseptic . Among the popular species, P. niruri, P. urinaria, P. emblica, P. flexuosus and P. amarus, have received the most attention. Phyllanthus amarus known as “Bhui amla” (Family – euphorbiaceae) widely distributed throughout the rainforest region of Northern India. It is an annual herb grows up to 15-60 cm high have an erect stem. The plant is most commonly used in the Indian Ayurvedic system of medicine in problems of stomach, genitourinary system, liver, kidney and spleen . P. amarus have different classes of organic compounds of medicinal importance including alkaloids, flavonoids, hydrolysable tannins (Ellagitannins), major lignans, polyphenols, triterpenes, sterols and volatile oil . MATERIAL AND METHOD: Collection & Authentication: The Phyllanthus amarus was collected from Chandrashekhar azad agriculture university, Kanpur in the month of July-August. The plant material was authenticated by Prof J. P. Shukla, Department of Botany, D.B.S College, Kanpur where a voucher specimen (PH/PA/20) is deposited for further reference. Correspondence to Author: